Prokaryotic expression system (E. coli expression system) is the earliest and most widely used classical expression system in protein expression technology. In recent decades, E. coli expression system has been continuously developed and improved, and has been widely used for various recombinant protein expression by scientific research and industrial users. Compared with other expression systems, it has the characteristics of high expression level of target gene, short culture cycle, strong anti-pollution ability and relatively low cost.
In more than 10 years of technical accumulation, Qiangyao Biology pays attention to details, improves the process, solves various technical problems in prokaryotic protein expression for customers, successfully delivers thousands of protein expression projects to customers every year, and provides strong technical support for scientific research customers.
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Competitive price, high cost performance.
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10 + years experience in protein expression, delivery success rate of over 96%.
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High standard delivery, the whole process of the integration solution
Service Items
Content
Deliverables
Delivery Date
The gene synthesis
Gene codon optimization and synthesis primer design, build T carrier, construct cloning bacteria
T carrier and sequencing report
1 to 2 weeks
Genes and cloning
Construction of expression vector, choose a suitable carrier
Expression vector and sequencing reports
1 week
E. coli expression strain transformation and filtering
Extraction build good expression plasmid into efficient DH5 alpha, BL21 (DE3), Rosetta (DE3), select suitable strains
The expression of recombinant plasmid strains and test report
1 week
The target protein expression and purification
The induction time, culture temperature, IPTG concentration and protein purification scheme were optimized
Delivery of the target protein, 95% purity
2 weeks
Provide protein folding method, in addition to the bacterial endotoxin, freeze-dried, large-scale purification, etc.
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